Original Articles: 2013 Vol: 5 Issue: 2
Standardization of callus induction in Saraca indica Auct, Non Linn
Saraca indica auct, non Linn commonly called as “Ashoka” is a vulnerable species, becoming rarer in its natural habitat. Callus culture was initiated from leaf, stem and flower bud explants of Saraca indica using Murashige and Skoog (1962) basal medium containing 3% sucrose and 0.8% agar supplemented with different concentrations (2, 3, 4 and 5 mgL-1) of 2,4-Dichlorophenoxy acetic acid (2,4-D). Leaf and stem explants produced 100 percent callusing in all the concentrations tried, whereas poor callusing response was observed with flower bud explant. Leaf explant was chosen for a detailed callus induction study with different concentrations and combinations of auxins 2,4-D and naphthalene acetic acid (NAA). Among the different treatments, leaf explant showed optimum callusing in cultures supplemented with 3 mgL-1 of 2,4-D alone. Treatments with NAA alone showed very poor callusing response. When combinations of 2,4-D (3 to 5 mgL-1) and NAA (3 to 5 mgL-1) were used, root formation was induced along with callusing. The fresh calli produced in all the treatments were white and hard which turned brown after two weeks.