The complex [CuL2]Cl2.H2O (where L = 1-amidino-O-ethylurea) has been synthesized and characterized. X-ray powder diffraction study reveal that the complex have orthorhombic crystal lattice. The unit cell parameters of the complex are a = 13.6766A°, b = 12.7978A°, c = 5.2467A°, and cell volume V = 918.35 A°. The DNA binding property of this complex has been investigated under physiological conditions (pH 7.2, 25±0.2oC) by absorption spectroscopy, cyclic voltammetry and viscosity measurements. The results indicate that the complex bind with CT DNA by non-intercalative mode with intrinsic binding constant in the order of 104M-1. Further, the complex has been screened for its antibacterial activity against Escherichia coli, Klebsiella pneumoniae sub sp. pneumoniae and Proteus mirabilis bacteria.