Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2012 Vol: 4 Issue: 3

Spectrophtometric method for the determination of artenimol using Rhodamine B as a new reagent


A Simple s e n s i t i v e a n d r a p i d spectrophotometric method for the determination of Artenimol is described. The method is based on the reaction of H2O2, generated by the cleavage of endoperoxide linkage of Artenimol and its reaction with potassium iodide to liberate iodine. The liberated iodine bleaches the colored Rhodamine B to colorless species and is measured at 550 nm. The Beer’s law is obeyed in the range of 33 – 274 μg/ ml for Artenimol using Rhodamine B as reagent. The Sandell sensitivity, detection limit and quantization limit were also calculated. The optimum reaction conditions and other analytical parameters were evaluated.