Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2013 Vol: 5 Issue: 5

Spectrophotometric estimation of oxolamine citrate in bulk and pharmaceutical dosage form by first order derivative and area under curve methods

Abstract

A simple and precise first order derivative and area under curve [AUC] UV- spectrophotometric methods have been developed and validated for the estimation of oxolamine citrate in bulk and its tablet formulation. The standard and sample solutions of oxolamine citrate were prepared in distilled water. Oxolamine citrate was estimated at 229.2 nm for the first order derivative UV-spectrophotometric method (A), while in area under curve (AUC) method (B) the zero order spectrum of oxolamine citrate was measured in between 228.6 nm to 246.4 nm. Beer’s law was obeyed in the concentration range of 1 to 14 μg / ml with coefficient of correlation value 0.9991 for first order derivative method. Similarly in AUC method, Beer’s law was obeyed in the concentration range of 1 to 14 μg / ml with coefficient of correlation value 0.9999. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation, which was within the range of 1.06915 % to 2.2714 % for the above two methods. The proposed methods were successfully applied for the determination of oxolamine citrate in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.