Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2015 Vol: 7 Issue: 3

Spectrophotometric determination of Tiopronin based inhibitory effect on hemoglobin


A highly simple and sensitive catalytic spectrophotometric method for the determination of tiopronin based on its inhibitory effect on the hemoglobin-catalyzed the reaction of H2O2 and acid chrome blue K(ACBK) was developed. The concentration of tiopronin is linear with the percentage inhibition (I%) of system under the optimal experimental conditions. The calibration graph is linear in range 0.05 to 2 mg/L with the detection limit of 8.5μg/L. The relative standard deviation was 4.1 % for 11 determinations of 1.5 mg/L. This method can be used for the determination of tiopronin in pharmaceutical preparations with satisfactory results.

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