A novel Antioxidant study of 6-(trifluoro methyl) furo [2, 3-b] pyridine-2- carbohydrazide derivatives, a sensitive spectrophotometric method was employed. The method is based on the reaction of these drugs as n-electron donors with the pi-acceptor 2,3,5,6-tetra chloro 1,4-nenzo quinone. The obtained coloured charge-transfer complex was measured at 440nm. The obtained complexes were confirmed by using the 1HNMR and IR spectral analysis. The proposed procedure could be applied successfully to the determination of the Free radical Scavenging activity of the title compounds. Among the Twelve analogs, V3 exhibited highest antioxidant activity and very low IC50 value. The association constants and standard free energy changes using Benesi-Hildebrand plots were studied.