Original Articles: 2015 Vol: 7 Issue: 6
Spectrophotometric absorbance correction method for the estimation of Tazobactam and Cefepime in combinedtablet dosage forms
A new, simple, precise, accurate and sensitive UV ÃƒÂ¢Ã‚â‚¬Ã‚Â Spectrophotometric absorption correction method has been developed for simultaneous determination of Tazobactam and Cefepimein combined tablet dosage form using 0.1 N NaOH as solvent. Absorbance correction method was based on the property of additivity of absorbances. The wavelengths selected for the absorption correction method were 259 nm and 306 nm. At 306 nm, Cefepime showed some absorbance while Tazobactam showed zero absorbance. Both the drugs gave absorbance at 259 nm. The method involved solving of an equation based on measurement of absorbance at two wavelengths 259 and 306 nm. The method was validated statistically. The determinations were made at 259 nm for Tazobactam and Cefepime and 306 nm for Cefepime over the concentration range of 3-18 μg/ml for Tazobactam and 10-50μg/ml for Cefepime with mean recovery of 100.34 ± 0.73 % and 99.89 ± 0.52 for Tazobactam and Cefepime, respectively by absorbance correction method. The precision for intra-day and inter-day of the method were found to be within the limits (RSD <2%). This method was found to be precise, accurate, simple, sensitive, reproducible and economical and can be applicable for the simultaneous determination of Tazobactamand Cefepime in combined dosage form.