Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2015 Vol: 7 Issue: 6

Simultaneous spectrophotometric estimation of Paracetamol and Aceclofenac by second order derivative method in combined dosage form


The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. second order derivative method for the determination of paracetamol and aceclofenac in combined dosage form i.e. tablets by using methanol as a solvent. The method was further validated by ICH guidelines. The proposed second order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 267 nm and 224 nm were selected for the estimation of paracetamol and aceclofenac respectively. The linearity of the proposed method was found in the concentration range of 1 to 12 μg /ml (r2= 0.9984) for Paracetamol and 1 to 14 μg /ml (r2= 0.9985) for aceclofenac respectively. The percentage mean recovery was found to be 99.580 % for paracetamol and 101.166 % for aceclofenac respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method.