Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

Reach Us reach to JOCPR whatsapp-JOCPR +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2015 Vol: 7 Issue: 7

Simultaneous spectrophotometric estimation of Aceclofenac and Thiocolchicoside by second order derivative method in combined dosage form


The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. second order derivative method for the determination of aceclofenac and thiocolchicoside in combined dosage form i.e. tablets by using methanol as a solvent. The method was further validated by ICH guidelines. The proposed second order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 215.1 nm and 278.2 nm were selected for the estimation of aceclofenac and thiocolchicoside respectively. The linearity of the proposed method was found in the concentration range of 1 to 14 μg /ml (r2= 0.9995) for aceclofenac and 1 to 14 μg /ml (r2= 0.9998) for thiocolchicoside respectively. The percentage mean recovery was found to be 100.255 % for aceclofenac and 100.108% for thiocolchicoside and respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method.