Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2013 Vol: 5 Issue: 5

Simultaneous estimation of statins like pravastain, atorvastatin and simvastatin in bulk and pharmaceutical dosage form by means of High-Performance Liquid Chromatography

Abstract

The aim of this study was to develop and validate a simple, rapid, precise, more accurate, reliable, least time consuming HPLC method for individual as well as simultaneous estimation of pravastatin, atorvastatin and simvastatin in bulk and pharmaceutical dosage form. The chromatographic separation was achieved by using a mixture of methanol and 0.1 % orthophosphoric acid in water as the mobile phase, with a C18 (150×4.6 mm i.d., 2.7 μm) reversed-phase column at flow rate of 1.0 mL/min and the eluents were monitered at 238 nm. A good linearity was found in the 0.12-0.24 mg/mL for both pravastatin and atorvastatin and 0.02-0.14 mg/mL simvastatin concentration range. The accuracy was good and recovery values for pravastatin, atorvastatin and simvastatin ranged from 99.21-100.40%, 99.87-100.39 and 98.84-100.66%, respectively. The proposed novel method was found to be efficient, accurate, precise, specific and economic and is suitable for individually as well as simultaneous estimation in quality control laboratories and research institutes.