Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2012 Vol: 4 Issue: 10

Simultaneous Determination of Moxifloxacin Hydrochloride and Dexamethasone Sodium Phosphate in Eye Drops by HPLC and Absorbance Correction Method

Abstract

High performance liquid chromatography and Absorbance correction methods were applied for the simultaneous determination of Moxifloxacin hydrochloride and Dexamethasone sodium phosphate in eye drops. Chromatographic separation was achieved on reversed-phase C18 column (25 cm × 4.6 mm id, 5 μm) in the isocratic mode using methanol-water-triethylamine (60:40:0.75, v/v/v; pH adjusted to 3.25 ± 0.05 with orthophosphoric acid) as the mobile phase at a flow rate 0.8 mL/min. Quantitation was achieved with UV detection at 254 nm. In the proposed HPLC method, the concentration range of 5-30 and 1-6 μg/mL, with mean recoveries of 101.46 ± 1.57 and 101.50 ± 1.64 % for moxifloxacin hydrochloride and dexamethasone sodium phosphate respectively was obtained. In the Absorbance correction method, quantification was achieved over the concentration range of 10-50 and 2-10 μg/mL, with mean recoveries of 100.49±0.62and 100.36 ± 1.09% for moxifloxacin hydrochloride and dexamethasone sodium phosphate respectively. Determination was performed at 244.2 nm and 357 nm for moxifloxacin hydrochloride and dexamethasone sodium phosphate. The proposed methods were successfully applied for the analysis of synthetic mixtures and pharmaceutical formulations of moxifloxacin hydrochloride and dexamethasone sodium phosphate without any interference from common excipients. The results obtained by applying the proposed methods were statistically compared by the Student’s t-test.