Original Articles: 2017 Vol: 9 Issue: 4
Simplified Extraction and Characterization of Acetic Acid Solubilized Type I Collagen Derived from Solea Solea Skin and Wistar Rat Tails for Biomedical and Biotechnological Applications
This research project presents an experimental plan to control the best possible processing parameters (acetic acid concentration, centrifugation speed and temperature) for the extraction of acetic acid soluble collagen (AASC) from the Wistar rat tails and the skin of Sole fish (Solea solea ). The results showed that these three independent variables had significant effects on the yield and the physico-chemical proprieties of AASC. Moreover, the extraction efficiency was also affected by the interaction between these variables. Optimal conditions for a higher yield of AASC extracted from rat tails, as well as the extracted from Sole fish, were: an acetic acid concentration of 0.1%, a centrifugation speed of 3000 rpm and an extraction temperature between 4 and 20°C. The characterization of AASC shows an acceptable pH value (3 to 5), an electrical conductivity and resistance variable relatively to the temperature and exponentially with acetic acid concentration. Relative viscosity of AASC dissolved in different acetic acid concentrations (0.1, 2.9 and 10%) decrease continuously as the temperature increase from 4 to 50°C, indicating a satisfactory degree of thermal denaturation of AASC molecules. Therefore, a skin of Sole solea, as well as Wistar Rat tail, can be used as alternative collagenous sources.