Zinc Vanadate (ZnV2O5) Nanocrystals were prepared by two steps, ceramic route process. Optimization of the ceramic route processing conditions enhances the Electrocatalytic and Antimicrobial performance of the prepared nanoparticles. The thermally treated material was subjected to XRD, FTIR, UV-Visible spectroscopy, SEM and EDS, which confirmed the formation of Zinc Vanadate (ZnV2O5) Nanocrystals. The electrochemical detection of Anthracene by Zinc Vanadate nanoparticles was investigated by Cyclic Voltammetry. The result concludes that the ZnV2O5 NPs have higher activity for detection and oxidation of Anthracene. The as synthesized ZnV2O5 NPs were found to exhibit strong antimicrobial activity against both Escherichia coli (E.coli) gram negative and Staphylococcus aureus (S. aureus) gram positive microorganisms implying their strong potential as antimicrobial agent.