Phytochemicals are secondary metabolites in one or more parts of the medicinal plants. These have the ability to produce a definite physiological action on the human body. Therefore present study was planned to evaluate TLC and GC-MS analysis of phytochemicals and in vitro antioxidant activity of the leaf extract of Euphoria hirta Linn. The phytochemical constituents were screened through TLC and GC-MS studies and the in vitro antioxidant activity of the alcohol and acetone extract was also investigated. The E.hirta has flavanoids, steroids and phenols which were screened through TLC and GC-MS analysis. In in vitro activity, the highest inhibitory activity of Euphorbia hirta was found in acetone extract when compared to alcoholic extract. The leaves of various extracts of Euphorbia hirta possessed lots of phytochemical constituents and have a potent in vitro antioxidant activity.