A total of 280 isolates were recovered from 100 milk samples. The samples were collected from the various dairies in Paonta Sahib. The isolates were characterized and identified on the basis of morphological characterization such as Gram staining reactions, colony characteristics and Biochemical test based on the Morphological and Biochemical characterization. It was observed that milk sample have the prevalence of E.coli sp. (32.14%), Staphylococcus sp. (21.4%), Salmonella sp. (21.4%), Proteus sp. (17.8%) and Bacillus sp. (7.14%) respectively. The Amoxycillin, Ciprofloxacin, Gentamicin, Ofloxacin, Ceftriaxome and Tetracycline were very sensitive to the Salmonella, Staphylococcus and Proteus. The Penicillin-G and Ampicillin were resistant to the E. coli and Proteus.