A simple, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic (RPHPLC) method was developed and validated for simultaneous estimation of Ofloxacin and Nitazoxanide (NTZ) on RP C-18 column (Shimadzu liquid chromatograph LC-10ATVP) using Tetrabutyl ammonium hydrogen sulphate: methanol: acetonitrile (20:20:60) as mobile phase at a flow rate of 0.5ml/min and the detection wave length was 280nm. The retention time for OFX and NTZ was found to be 4.67 and 7.79 min respectively. The recovery was greater than 98% with RSD less than 1.00. The proposed method was validated for precision, accuracy, linearity, range, robustness and ruggedness.