A simple, rapid, sensitive, reverse phase isocratic RP-HPLC method was developed for determination of Valsartan in tablet dosage form. The method was carried out using Thermohypersil ODS column (150 mm × 4.6 mm i.d., 5 μm particle size) with mobile phase comprised of water: acetonitrile: glacial acetic acid (500:500:01). The flow rate was set at 1.0 ml/min and effluent was detected at 273nm. The retention time of valsartan was found to be 4.6 minute. The method was validated for specificity, accuracy, precision, linearity, and limit of detection, limit of quantification, robustness and solubility stability. LOD and LOQ were found to be 2.72 μg/ml and 8.25 μg/ml respectively. The calibration curve was linear in the concentration range of 40- 140 μg/ml with coefficient of correlation 0.9990. The percentage recovery for the valsartan was found to be 99.0-100.2 and the % RSD was found to be less than 2 %. The proposed method was successfully applied for quantitative determination of valsartan in tablet dosage form.