Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 5

RP-HPLC method development and validation for the determination of ciprofloxacin from marketed tablet dosage forms


The present study deals with the development and validation of a simple, rapid, precise and economical reverse phase liquid chromatographic technique for the determination of Ciprofloxacin form marketed tablet dosage forms. Chromatographic analysis was carried out using C18 Xterra Column (150 × 4.6 mm, 5µm) and mobile phase consisting of a mixture of 5.3mM phosphate buffer (pH = 3.5) and acetonitrile in the ratio 60:40 at a flow rate of 0.5ml per minute and the detection was done at 275nm. The retention time of analyte peak was 2.29 min. The linearity was obtained in the concentration range of 0.01 to 0.50 µg/ml with correlation coefficient 0.99. The analysis results of ciprofloxacin were validated statistically and by recovery studies and the mean recovery was found to be 100.14%. The limit of detection (LOD) and limit of quantification (LOQ) for ciprofloxacin was found to be 0.06 µg/ml and 0.20 µg/ml, respectively. The proposed procedure was found to be accurate and precise for the estimation of ciprofloxacin from marketed formulations and can be used for the regular analysis of this drug in quality control laboratories.