Donepezil hydrochloride is used in the Alzheimer’s disease used in neurodegenerative disorder characterized by progressive attribute some of them to a deficiency cholinergic neurotransmission. Its therapeutic effect by enhancing cholinergic function it was accomplished by increasing the concentration of acetyl cholinesterase through reversible inhibition of its hydrolysis. High-performance liquid chromatography is the fastest growing analytical technique for analysis of drugs. Its simplicity, high specificity and wide range of sensitivity make it ideal for the analysis of many drugs in both dosage forms and biological fluids. The Donepezil hydrochloride is used as a active ingredient in the pharmaceutical dosage form. Pharmacopoeias have not yet provided an official method for its quantization. Hence present aim of the work is to develop a specific. precise, accurate, linear, simple, rapid and cost effective analytical method for Donepezil hydrochloride in tablet dosage form by RP-HPLC and UV spectrophotometric method. The work extends to validate for the developed methods as per ICH guidelines.