Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 9

Rifamycin SV production using immobilized cells of Amycolatopsis mediterranei OVA5-E7 in different matrices

Abstract

Rifamycin SV is abroad-spectrum antibiotic widely used in human drug therapy. Cell immobilization can enhance the productivity of secondary metabolites such as antibiotics. The purpose of the present work is to enhance the yields of rifamycin by immobilization of mutant strain of Amycolatopsis mediterranei OVA5-E7.Calcium alginate was found effective and suitable for improving the productivity of rifamycin SV under repeated batch fermentation compared to other matrices. Rifamycin SV production with immobilized A.mediterranei cells was better matric made of 2% alginate and 3% calcium chloride with 1h curing time and maintained at pH 7.0 while the temperature was maintained at 28ºC for a period of 6 days. The rifamycin SV production increased to 6396 mg/L which yielded 1.5 folds higher quantity of antibiotic than free cells cultured under similar conditions.The cell leakage from the Caalginate matric is 0.94 mg/ml enabling five repeated batch fermentations with each batch lasting for 6 days.Similarly, the yields from other immobilization matrices are 4865mg/L in k-Carrageenan, 4620 mg/L in agaragar, 4552 mg/L in polyacrylamide and 4401 mg/L using gelatin.Compared to earlier scientific reports, Ca-alginate entrapped cells of Amycolatopsis mediterraneia long with fine-tuned fermentation parameters as reported here were found effective for repeated batch submerged fermentations towards maximizing the rifamycin SV yields.