Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2015 Vol: 7 Issue: 1

Reversed phase high performance liquid chromatography method for determination of carvedilol hydrochloride from active pharmaceutical dosage form

Abstract

A simple, rapid and accurate high performance liquid chromatography method is described for determination of carvedilol hydrochloride from active pharmaceutical ingredients. The separation of drug was achieved on BDS hypersil C18 (150 x 4.6 mm i.d.) with 5 μ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of a mixture of buffer and acetonitrile (70:30 % v/v). The buffer was mixtures of 0.01 % tri-ethyl amine adjusted the pH 3.3 with ortho-phosphoric acid. The detection was carried out at wavelength 240 nm. The mixture of water and acetonitrile (50:50% v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze carvedilol hydrochloride from active pharmaceutical ingredients.