Alzheimer’s disease (AD) is a potential neurodegenerative disorder of nervous system which affects the primary areas of the brain dealing with learning and memory. Deposition of the insolublebeta-amyloid protein on neurons activates the microglial cells which stimulates the process of neuroinflammation by releasing mediators. Release of cytokines and apoptotic promoters in turn shrink the neurons of the hippocampus leads to cognitive impairment. In the present study change in histology of hippocampi neurons in mice brain during AD will be evaluated by intra cerebro ventricular (ICV) injection of beta-amyloid 25-35.Toluidine blue, haematoxylin and eosin (H&E) staining methodswereutilized for analyzing the neuronal morphology of Cornuammonis (CA) and dentate gyrus (DG) zones of hippocampus. Results obtained from the histopathological study reveals that decrease in the number of hippocampi neurons may be due to degeneration. Presence of darken condensed nucleus, shrinkage of pyramidal cells in CA zones and decrease in granular cells of DG zone is a strong indication of apoptosis and stress cause by amyloid injection. Hence it was concluded that cognitive impairment caused by beta-amyloid in mice is mainly due to degeneration of neurons in functional zones of hippocampus that controls the memory and learning behavior.