Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2011 Vol: 3 Issue: 6

Resistant bacteria a threat to antibiotics


Objectives: The study was conducted to characterize the citywide epidemiology of ESBLs in Hail, to detect the frequency and types of enzymes by determining whether the responsible genes are spreading among different strains, or whether one or few strains are account for the spread of these enzymes among the local population. Methods: One hundred urine samples were collected and screened at local hospital during July- Aug 2011.The E.coli isolates resistant to third generation cephalosporins (ceftazidime). The CTX-M genes were detected by PCR and the RAPD typing done. E-Test was performed on 30 selected isolates and were reconfirmed by disc diffusion method for the confirmation of resistance. Results: Polymerase chain reaction (PCR) was used to detect the ESBLs genes. The collection of ESBLs positive isolate from the hospital and community for CTX-M were 78. Twenty two isolates did not contain blaCTX-M which may contain blaTEM ,SHV or OXA genes (not tested). The overall prevalence of isolates of E.coli was high. ESBLs isolates were found in both the community and hospital, with the CTX-M type most common.