With economic development and increasing abundant agricultural products, consumers’ demand for agricultural products has changed a lot, but attention to quality safety of agricultural products is also increased, while agricultural leading enterprise is the mainstream of agricultural product processing, and management level of food safety is related to agricultural products safety level as well as to consumers’ health. Opportunism behavior occurs in the process of agricultural leading enterprise production due to bad management, and finally makes consumers’ consumption behavior different consumption intention. The research adopts random sampling method from developed provinces of domestic agricultural leading enterprises to research on mutual relation and impact of food safety supervision method, opportunism behavior and consumer behavioral intention of agricultural leading enterprise in the method of regression analysis and correlation analysis. Research results have significant theoretical and realistic significance on improving food safety supervision level and consumers’ health in China agricultural leading enterprises.