This paper investigated the optimum microwave-assisted extraction proeess of Schisandra chinensis by application of orthogonal tests. Taking the antibacterial activity as index, with extraction time, extraction power, solvent and solid-liquid ratio as factors, extraction technology was optimized by L9 (34) orthogonal test. By using the mycelium growth rates method, the inhibition of Schisandra chinensis extracts were measured, the lethal concentration EC50 were calculated. It was found that the optimum extraction technology were: microwave power at 400W, extraction time at 5 min, solid-liquid ratio is 5:70 and solvent is acetone.