The bio-sorbents derived from leaves of Achyranthes aspera, Hsibisuc rosa-sinensis and Tribulus terrestris have been studied for their sorption nature towards ammonia from polluted waters with respect to various physicochemical parameters such as pH, sorbent concentration and equilibration time. The conditions for maximum removal of Ammonia from polluted waters have been optimized. The interference of common anions and cations that present in natural waters on the % of extraction of ammonia under optimum conditions has been studied. It is found that anions like Chlorides, Fluorides, Sulphate, Phosphate and Carbonates have marginal interferenc while Cations like Ca2+, Mg2+, Cu2+, Zn2+and Ni2+ interfered to some extent. The adoptability of the methodologies developed in this work are tested with diverse samples collected from industrial effluents and naturally existing polluted lakes and it is found that the procedures are remarkably successful in removing the substantial amounts of ammonia from waste waters.