An analytical evaluation strategy, based on quantitatived determination of loureirin A and loureirin B in Dragon’s blood capsules from five manufacturers by RP-high performance liquid chromatography. The samples were extracted with methanol by the ultrasonic extraction method and separated on a Kromasil C18 (5 μm, 4.6×200 mm) chromatographic column by using acetonitrile–0.1% acetic-acid (37: 63) as mobile phase with the detection wavelength at 280 nm. The effects of experimental conditions, such as extraction method, column temperature, and different type of chromatographic column, on separation efficiency were investigated. Under the optimal conditions, the main ingredients of loureirin A and loureirin B in dragon's blood capsules reached the baseline separation. The method showed good linearity with correlative coefficient of 1.0000. The recoveries were 98.07%~101.29%, and the relative standard deviations were 0.29~0.45%. The developed method can be adopted for the determination of loureirin A and loureirin B in dragon's blood capsules of five manufacturers. The results showed that there was remarkable difference in different origin of Dragon’s blood.