To investigate the quantification of total phenolic and in-vitro antioxidant activity of distilled water, ethanol, butanol and chloroform extracts of A. rosea seeds. The quantification of the total phenolic content was estimated by taking gallic acid as a standard. Antioxidant activities were studied via different methods that including DPPH assay, superoxide anion scavenging assay, hydroxyl radical scavenging assay and superoxide dismutase activity assay. The results showed that the distilled water extracts of A. rosea seeds have more phenolic content than other extracts. The four solvent extracts were found to possess concentration dependent scavenging radical’s activity. With current findings, A. rosea seeds might be used as potential natural antioxidant.