Lichen cells from the thalli of Stereocaulon ramulosum were maintained with kaolinite and sodium acetate as precursor of phenolic biosynthetic pathway in three different systems: continuous flow, fixed and continuous agitation. Fractions were collected and monitored by TLC and spectrophotometry, showing increases in the concentration of metabolites produced by the cells, being the most effective method continuous flow. The cells showed vitality after submission in liquid medium, and capable of producing. This methodology shows the advantage of being economical and effective for the production of substances of pharmacological interest from lichens without causing environmental damage or collection of large amounts of material for obtaining significant amounts of substances.