Urinary tract infections (UTIs) are common bacterial infections in children. The present study was undertaken to evaluate the trend of Antimicrobial agents (AMAs) prescription in children with UTI and also the sensitivity patterns of the uropathogens isolated in our hospital. 100 in-patients satisfying the inclusion criteria were selected. About 38.8% of the patients were in the age group of 1-5 years and 46% of the patients received AMAs for 6-10days. E.coli mainly sensitive to Amikacin, Nitrofurantoin and resistant to Norfloxacin, Ciprofloxacin was isolated in majority of the urine samples in our study. Most commonly prescribed drugs in complicated UTI (cUTI) was Amikacin (19) in the hospital and Cotrimoxazole in simple UTI. The number of drugs per prescription was 5.2±2.4. The number of antibiotics per prescription was 3.5±1.5. The number of drugs prescribed by generic name was 35% and number of drugs prescribed from the WHO Model List of Essential Medicines was 96%. The prescription of AMAs were in accordance with guidelines from Indian paediatrics Nephrology group. In view of development of increasing resistance to fluoroquinolones, its empiric use in the treatment of UTI in children should be strongly discouraged. The number of antibiotic prescriptions and its intravenous administration could be reduced in our hospital by implementing a regional antibiotic policy.