The 9-fluorenone Schiff base derivatives have reported for the variety of diseases such as anti microbial and cancers. In this current study various Schiff bases were synthesized and characterized from 9-fluorenone.many compounds shows good in yield at specific temperature. Further the synthesized compounds were studied for anti microbial activity and molecular docking for Proteus mirabilis catalase enzyme and found that N,N'-Bis-fluoren-9- ylidene-ethane-1,2-diamine shows highest dock score of 81.947 with Internal energy (-7.169)and its microbial activity value as 17.9mm compared with other compounds