Original Articles: 2011 Vol: 3 Issue: 1
Preparation of Schiff base Zinc Metal complex (DMAPIMP)2Zn and Development of HPLC Chromatographic method for its analysis
A rapid, sensitive and specific RP-HPLC method involving UV detection was developed and validated for determination and quantification of (DMAPIMP)2Zn . Chromatography was carried out on Waters 2695 separation module HPLC system with Waters 2487 Dual wavelength Absorbance detector and Waters 2998 Photodiode Array Detector using a stainless steel column of dimension 15 cm x 4.6 mm , packed with octadecylsilane bonded to porous silica ( Make : Phenomenex Prodigy 5 μ ODS 3 100A column is suitable ) using filtered and degassed mixture of Buffer ( Prepared by addition of 1 L purified water and 10 ml Acetic Acid and 5 ml Triethylamine ) and Acetonitrile ( 60:40) as a mobile phase at a flow of 1.0 ml/min and effluent was monitored at 254 nm . The method was validated in terms of Specificity, Linearity and Range, Precision, Accuracy, Intermediate Precision, Solution Stability and Robustness. The assay was linear over the concentration range of (DMAPIMP)2Zn 0.05 mg/ml to 0.15 mg/ml respectively. Accuracy of the method was found to be 98.98 % - 101.22 % within precision RSD of 1.33. The system suitability parameters such as theoretical plates and tailing factor were found to be 6325 and 1.14 respectively. The method requires only 8 minutes as run time for analysis which prove the adoptability of the method for the routine quality control of the drug.