Collagen was extracted from pig skin, and mixed with antioxidants, a moisturizing agent, and a crosslinking agent to prepare anti-aging face mask. The antioxidant activity of olive oil and tea polyphenols (TP) was studied using DPPH• assay. The results show that both olive oil and TP present a clearance effect towards DPPH• radicals. The best clearance effect was obtained when the ratio of olive oil to TP is 1g: 0.6mg. Single factor experiment shows that when the amount of added polyvinyl pyrrolidone was 0.03 g, the antioxidants can be uniformly dispersed in the film. The optimal formulation and processing parameters were obtained for the fabrication of anti-aging face mask from collagen, olive oil, tea polyphenols, lactic acid and glutaraldehyde. The resulting collagen anti-aging face mask present outstanding moisturizing, repairing, whitening, anti-oxidation, anti skin aging effects.