Morinda pubescence, one of the important plants of Rubiaceae family, is a small tree distributed through out the tropics. It is one of the most important species used in Ayurveda for the treatment of different diseases. The plant parts are used as cathartic, febrifuge, healing of ulcers, throat complaints, dysentery, leucorrhoea and sapromia and tonic. Amino acids play a vital role in the metabolism of secondary metabolites. Taking into consideration the above facts the chemical analysis of various extracts of the leaves was performed to detect the presence of amino acids. The amino acids were identified by paper chromatographic method using varied solvent systems. The presence of amino acids was determined by comparing with standard amino acids.