Oxystelma esculentum distributed in the southern part of India particularly in Tamilnadu has potential medicinal properties and it is used as diuretic, anthelmintic, oxytocic and laxative. Phytochemical screening of leaves extract revealed the presence of alkaloids, tannins, steroids, saponins, terpenoids, flavanoids, glycosides and phenolic compounds. The aim of this study was to screen the phytochemicals present in the leaf of Oxystelma esculentum and further analysis of the components present in antimicrobial activities. The microorganisms gram positive and gram negative bacterial viz. Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Pesudomonas aeruginosa and Proteus vulgaris and fungal viz. Aspergillus niger, Aspergillus flavus and Candida albicans. The extracts of height activities of Staphylococcus aureus.