‘Nirgudi’ plant Vitex negundo Linn. from family Verbenaceae is important medicinal plant in Indian Pharmacopoeia with variety of bioactive phytochemicals having importance in pharmacology and tribal medicines. It was found to contains many polyphenolic compounds, terpenoids, steroids, glycosides and alkaloids. Bioactivity guided preliminary phytochemical analysis of various extracts of leaves of V. negundo resulted in the qualitative analysis of ten metabolites. All the crude extracts from various solvents were evaluated for their antibacterial properties. They were found to have significant antibiotic activity against Staphylococcus aureus, Klebsiella pneumoniaee, Escherischia coli, Pseudomonas aeruginosa, Salmonella typhi and Bacillus subtilis. The significance of antibacterial activity was compared with standard antibiotic tetracycline.