Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2018 Vol: 10 Issue: 6

Preclinical Study on Expression of Bax and Bcl-2 Apoptotic Regulatory Proteins on Amyloid Beta (A?² 25-35) Induced Oxidative Stress in Alzheimerâ??s Mice

Abstract

Alzheimer’s disease (AD) becomes global threat as it prevails very rapidly in the developing countries like India. Progression of AD predicted majorly by deposition of protein called amyloid beta which is a hallmark protein majorly responsible for triggering most of the inflammatory episodes in the etiology of the AD. Chronic inflammation and oxidative stress ultimately contributes to the neural cell death which in turn leads to galvanizing of caspases. Being a protease enzyme caspases constantly involved in fragmenting the core cellular components such as DNA and other functional proteins in the neurons essential for learning and memory. Proportion of bcl-2 protein (Anti-apoptotic) with respect to Bax protein (Pro-apoptotic) regulates the survival or death of the neuronal cell after apoptotic stimuli in amyloid induced neurodegenrated mice model. Ipomoea aquatic Forsk is a novel medicinal herb traditionally used for treating nerve debilities throughout India but still now there is no proper documentary evidence available on its molecular action against neurodegeneration. This prompted us to peruse the present investigation on this versatile herb. The main aim of the present study is to determine the effect of hydroalcoholic extract of Ipomoea aquatica (HAEIA) on protein expression of anti-apoptotic BCL-2 gene and a pro-apoptotic BAX gene in amyloid beta (Aβ 25-35) induced Alzheimer’s mice. Swiss albino mice were treated with HAEIA for periods of 4 weeks dose-dependently (200 and 400 mg/kg) then received a single intra cerebro ventricular (i.c.v) injection of Aβ25-35 (10 μg/mouse). At the end of the study animals were sacrificed and their brains were isolated to study the expression of Bax and Bcl-2 gene by PCR technique. Results of the present investigation clearly projects that brain sample of mice treated with HAEIA has shown significantly increased level of expressing anti-apoptotic protein Bcl-2 further it was observed that there is remarkable decease in the expression of pro-apoptotic protein gene Bax and this become an evidence based data that the herb Ipomoea can able to limit the progression of apoptosis caused by amyloid injection. From the data’s of the present investigation it was concluded that the herb Ipomoea aquatic may serve as an valuable lead for the effective treatment and clinical management of neurodegenerative disorders like AD in near future.