Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 5

Polymer monolithic rods microextraction coupled with high performance liquid chromatography for the analysis of trimethoprim, sulfadiazine and sulfamethoxazole in honey samples


Novel polymer monolithic rods were prepared by in situ polymerization in a glass capillary and then coupled with high-performance liquid chromatography (HPLC) for the analysis of trimethoprim, sulfadiazine, and sulfamethoxazole. The rods showed homogeneity, uniform surface and micropores, good solvent-resistant ability, and excellent adsorptive ability onto antibacterial synergist compounds and sulfonamides. The extraction conditions were optimized, including extraction solvent, desorption solvent, extraction time, desorption time, and shaking frequency. A method of determining trimethoprim, sulfadiazine, and sulfamethoxazole through polymer monolithic rods microextraction coupled with HPLC was developed. The linear range was 20 μg/L to 200 μg/L, and the detection limits ranged within 10.6 μg/L to 15.5 μg/L. The proposed method was successfully applied to trimethoprim, sulfadiazine, and sulfamethoxazole analysis in spiked honey samples.