Acorus calamus has been used for centuries as a folk medicine to treat infectious and noninfectious diseases. Extracts of dried acorus prepared with different solvents, (acetone and ethyl acetate) were tested for Phytochemical analysis, antioxidant, antimicrobial, antioxidant, anticancer and dna polymerase inhibitory activity. Phytochemical analysis revealed that acetone extracts contains total polyphenolic and flavonoids 10.00 and 0.725 mg/gm where as tannic acid (35.55), Pyrogallic acid(694.8), and Querctin (428.8) ug/gm. Antioxidant activity of acetone and ethyl acetate crude extracts by FRAP are 47.03 and 30.09Mm FeSO4 / gm of sample. Acetone extract is having potent antimicrobial activity against S.Typhi, Ecoli and M. Luteus are 47, 43.85, 100 % inhibition at 13 ug conc. of extract. Complete DNA polymerase inhibition observed at 6.66 ppm conc. of acetone extract where no inhibitions observed in a ethyl acetate extract. Acetone extract is having high HT – 29 cell proliferation inhibition activities (53.44% inhibition) 10 ug of extracts. Acorus calamus is beneficial for the future multi diseases therapeutic research study.