The main objective of this present work is to find out good pharmacological activities in herbal source with their phytochemical study, which is aimed to investigate of 80% ethanolic extract of Dodonaea Viscosa flowers for their antioxidant, analgesic and cytotoxic activities. Fractionation of this extract led to the isolation of fourteen natural bioactive compounds, among them a novel one was isolated and identified as; 6-OH-kaempferol-3,6-dimethylether- 4`-glucopyranoside besides 13 known compounds including one kaempferol glycoside, three methylated kaempferol,onequercetin glycoside, five methylated quercetin and three aglycones. All the pure compounds were identified by chromatographic methods, chemical degradation and spectral data (1H NMR, 13C NMR). The 300 μL ethanolic extract concentration showed the most potent antioxidant activity with inhibition % 82.09 ± 0.15 using the DPPH radical scavenging method which is higher than the standard BHA (68.2) and lower than the standard TBHQ (98.2). Its cytotoxic activity against breast carcinoma cell line (MCF7) showed IC50 19.4 μg/ml compared with the standard used (Cisplatin), which its IC50 is 5.48μg/ml. Its anti-nociceptive properties showed that the 200 μL ethanolic extract concentration significantly (p<0.05) increased the response time in hot plate method and decreased the number of writhes in acetic acid writhing test when comparing with the control group.