Phytochemical study and antimicrobial activity of Anthocleista djalonensis was carried out by solvent extraction using ethanol and aqueous solvents. Results obtained showed that both ethanolic and aqueous extracts contain alkaloids 4+_8%, tannins 1+_1%, flavonoids 3+_3%, cardiac glycoside 2+_3%, saponin 4+_5% and reducing sugar, while protein was absent in ethanol extract but present in aqueous extract. The antimicrobial screening should inhibit growth of staphylococcus aureus at 10.17+_0.74, eschericha coli 13.30+_ 0.22 and candida albicans 14.97+_0.17 for ethanolic extract, aqueous extract inhibition is from 7.07+_0.14 for staphylococcus aureus, 9.23+_0.12 for Eschericha Coli and 12.63+_ 0.31 for candida albicans .these are indication that the plant is used as medicinal plant.