The paper presents the results of phytochemical screening, antioxidant, antimicrobial and anti-proliferative activity studies of Arbutus pavarii (A. pavarii) plant. The whole aerial part of the plant was collected during the spring season (2010), identified and extracted with methanol, chloroform and n-Hexane. Phytochemical screening was carried out using standard procedures. Antioxidant activity was done using 2, 2, Diphenyl-1-picryhydrazyl (DPPH) assay. Antimicrobial evaluation was performed using agar well diffusion method. The anti-proliferative activity was evaluated on two breast adenocarcinoma and lung carcinoma cells. Phytochemical screening showed the presence of flavonoids, tannins, glycosides, simple phenolics, free reducing sugers, triterpenes and sterols in the plant extracts. The methanolic and chloroform extracts exhibited a potential antioxidant activity with IC50 of 4.55±1.90 μg/ml and 21.55±1.1 μg/ml in comparison to Quercetin. Methanolic extract exhibited antimicrobial effect against Staphylococus aureus, Escherichia coli and Candida albicans with zones of inhibition of 20mm, 18 mm and 21 mm and the minimum inhibitory concentrations (MICs) were 4.8 mg/ml, 69.30 mg/ml and 4.76 mg/ml using Ciprofloxacin and Amphotericin B as standards. The anti-proliferative activity of extracts with IC50 (less than 30 μg/ml) against breast adenocarcinoma (MCF7) and lung carcinoma (A549) was within the limits of the American National Cancer Institute for cytotoxic activity. A. pavarii may be exploited for the search of new antimicrobial and anticancer drugs.