The phytochemical screening, antioxidant and antibacterial activities of extracts from the flowers of Neoglaziovia variegata were investigated. It was also evaluated the total phenolic and flavonoid contents by the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using of 2,2- diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching and compared with ascorbic acid, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) used as reference compounds. The antibacterial effect was evaluated by the method of microdilution. Preliminary analysis demonstrated that the extracts were found to be positive for the presence of anthracene derivatives, flavonoids, tannins, mono and diterpenes. The most significant total phenolic content was of 249.63 ± 2.79 mg of gallic acid equivalent/g for chloroform extract (CHCl3), and 242.59 ± 12.64 for ethyl acetate extract (AcOEt). The total flavonoids content was of 483.31 ± 50.65 mg of catechin equivalent/g for CHCl3 extract. The ethanolic extract presented the best antioxidant activity (IC50 5.97 ± 0.22 μg/ml) for DPPH scavenging. BHA was the most effective antioxidant. Antioxidant activities and total phenolic contents were found to be highly correlated. The ethanolic extract showed activity against most of the microorganisms tested, especially Bacillus cereus, Enterococcus faecalis and Staphylococcus aureus.