The purpose of this research was to screening of phytochemical and antioxidant from seeds extract of water plant (Nymphaea stellata and Nelumbo nucifera). A powdered sample of the seeds of Nymphaea stellata and Nelumbo nucifera were extracted with ethanol to produce a crude extract. Phytochemical result of extracts confirmed the distribution of tannins, saponnins, and flavonoids. The antioxidant activities (IC50) of the extract of Nymphaea stellata and Nelumbo nucifera were 43.21 ppm and 139.84 ppm, respectively.