Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 8

Phytochemical and antimicrobial activity of fruit pulp of aegle marmelos

Abstract

The present investigation was carried out to evaluate the phytochemical constituents and antibacterial activities of fruit pulps of Aegle marmelos (Linn.) Correa. The phytochemical screening of the crude extract revealed the presence of Reducing Sugars, Saponins, Tannis, Flavonoids and Phenols. Further the total phenolic and flavonoid content was also estimated. Besides, the crude extract was tested for antimicrobial activity against two gram positive strains of Staphylococcus aureus (ATCC 29213, ATCC 700699) at different concentrations of 10, 50, 100, 250 and 500ug/ml at different time span of 3hrs. It was found that a concentration range 50-100ug/ml of the ethanolic extracts was effective in inhibiting the growth of bacterial strain Staphylococcus aureus ATCC 29213. 250ug/ml was effective for aqueous extract and 500ug/ml concentration was effective for petroleum ether extracts in inhibiting the growth of the above strain. When the similar study was carried out using other strain, Staphylococcus aureus ATCC 700699, it was found that 100ug/ml of ethanolic extract, 250ug/ml and 500ug/ml of petroleum ether was effective in inhibiting the growth of bacteria,where as the concentration of aqueous extracts taken were ineffective against the growth of the bacteria. The broad spectrum antimicrobial activity of fruit pulp extracts of Aegle marmelos was indicated by the inhibition of growth of the bacteria, which in turn in future, might lead to further study for the development of novel bioactive antimicrobial agents.