The present study was carried out to characterize the bioactive constituents present in plant extract of M. spicata using UV-VIS, FTIR and GC/MS technique. The plant extract was scanned in the wavelength ranging from 300- 800nm by using UV-VIS spectrophotometer and the characteristic peaks were detected. The UV-VIS profile showed different peaks ranging from 300-800nm with different absorption respectively. The FTIR spectrum confirmed the presence of alcohols, phenols, alkanes, alkenes, carbonyl, carboxylic acids and aromatic compounds in extract. The results of the GC/MS analysis of methanolic plant extract provide different peaks determining the presence of 42 phytochemical compounds. The major phytoconstituents were pentadecanoic acid (7.47%), 7- oxabicyclo[4.1.0]heptane (9.56%),3-penten-2-one,4-(2,2,6-trimethyl-7-oxabicyclo[4.1.0] hept-1-yl)-,(E)-(12.20%), Stigmast-4-EN-3-one (18.99%). The results shows that important bioactive compounds are present in plant extract and these constituents may be responsible for pharmacological activities.