Pineapple is a rich source of ascorbic acid supplement to our diet. Pineapple pulp waste showed maximum amount of reducing sugars(30.5mg/100g) and ash(1.8mg/100g)respectively. Pineapple waste contains high concentration of biodegradable organic material and suspended particles. Pineapple waste showed higher concentration of crude fibre, non-reducing sugar, protein, ascorbic acid and moisture content. Therefore pineapple waste is used as substrate for growth of microbes in fermentation process