Rabeprazole sodium (RSM) is a proton pump inhibitor used against peptic ulcer disease to suppress excess acid secretion in the stomach. Physico chemical characterization studies showed that RSM has showed a melting point of 1370 C. The solubility of drug RSM followed the order distilled water> pH 9.0> pH 8.0> pH 7.5> pH 2.5. The analytical method developed for the estimation of RSM in bulk fluids showed maximum absorbance λmax of 272.2 nm in distilled water between 200 nm and 400 nm. Linearity studies indicated that estimation of RSM between 2.00 μg /ml to 10.00 μg /ml was found to be linear with regression equation of y = 0.034*X -0.00267; ( r2 = 0.99961). The accuracy, precision studies showed that the recovery of drug from bulk fluids and dosage form are highly accurate and precise with minimum error. The above analytical parameters indicated that the developed UV Spectrophotometric method for RSM was simple, accurate, precise and reproducible.