Rabeprazole sodium (RSM) is a proton pump inhibitor used against peptic ulcer disease to suppress excess acid secretion in the stomach. Phys ico chemical characterization studies showed that RSM has showed a melting point of 137 0 C. The solubility of drug RSM followed the order distilled water> pH 9.0> pH 8.0> pH 7.5> pH 2.5. Th e analytical method developed for the estimation of RSM in bulk fluids showed maximum abs orbance λ max of 272.2 nm in distilled water between 200 nm and 400 nm. Linearity studies indica ted that estimation of RSM between 2.00  g /ml to 10.00  g /ml was found to be linear with regression equati on of y = 0.034*X -0.00267; ( r 2 = 0.99961). The accuracy, precision studies showed that the recovery of drug from bulk fluids and dosage form are highly accurate and precise wit h minimum error. The above analytical parameters indicated that the developed UV Spectrop hotometric method for RSM was simple, accurate, precise and reproducible.