Aqueous extract of C. tinctorius flowers is under consideration for development into Phytopharmaca for immunostimulant supplement. This study aimed to find the nonspecific and specific standard as a reference parameter extraction repeatability. Physicochemical determinations, including proximate analysis were done by sensory examination and gravimetric, thin layer chromatography with F245 silica gel using various solvent systems, metallic content analysis by AAS and pesticide residue by HPLC. The extract was dry but hygroscopic and soluble in water, with a loss drying at 6.0-6.7 %w/w, total ash at 4,92 – 5,37%w/w, acid insoluble ash at 0,506 – 2,89%w/w, water soluble extract at 10.36 - 11.90 %w/w and ethanol soluble extract at 10.00 - 10.54 %w/w. The extracts yielded reproducible chromatograms on normal silica plates developed with various solvent systems. Metallic level including cadmium at 0.0015-0.0058 μg/g, lead at 2.912 - 6.01 μg/g but arsenic no detected. Aldrin, dieldrin and endrin as pesticide residue no detected. Phenolic total at 3.8-4.8 %b/b expressed as tannic acid. The results confirm that aqueous extract of C. tinctorius flowers possess characteristics that would allow it’s possible development into Phytopharmaca and that meets standardization herb requirement.